Product name: Rat BK(Bradykinin) ELISA Kit
Catalog number: E-EL-R0124
Size: 96T
Reactivity: Rat
Detection Range: 1.25~80 ng/mL
Sensitivity: 0.75 ng/mL
Lead Time: 7~10 days
Intended use
This ELISA kit can be applied to the in vitro quantitative determination of Rat BK concentrations in serum, plasma and other biological fluids.
Test principle
This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Rat BK. During the reaction, Rat BK in the sample or standard competes with a fixed amount of Rat BK on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat BK. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat BK in the samples is then determined by comparing the OD of the samples to the standard curve.
Specificity
This kit recognizes some recombinant and natural Rat BK. No significant cross-reactivity or interference was observed.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Rat BK were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Rat BK were tested on 3 different plates, 20 replicates in each plate.
Kit components & Storage
The unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions since the kit is received.
Item |
Specifications |
Storage |
Micro ELISA Plate(Dismountable) |
8 wells ×12 strips |
-20℃, 6 months |
Reference Standard |
2 vials |
Concentrated Biotinylated Detection Ab (100×) |
1 vial, 120 μL |
Concentrated HRP Conjugate (100×) |
1 vial, 120 μL |
-20℃(shading light), 6 months |
Reference Standard & Sample Diluent |
1 vial, 20 mL |
4℃, 6 months |
Biotinylated Detection Ab Diluent |
1 vial, 10 mL |
HRP Conjugate Diluent |
1 vial, 10 mL |
Concentrated Wash Buffer (25×) |
1 vial, 30 mL |
Substrate Reagent |
1 vial, 10 mL |
4℃(shading light) |
Stop Solution |
1 vial, 10 mL |
4℃ |
Plate Sealer |
5 pieces |
|
Product Description |
1 copy |
Certificate of Analysis |
1 copy |
Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution.
The volume of reagents in partial shipments is a little more than the volume marked on the label, please use in measuring instead of directly pouring.